Název:	Effect of the Ionizing Radiation Dose on Histone H2AX
Autoři:	Skálová, Jana Vlas, Tomáš Mašata, David
Citace zdrojového dokumentu:	SKÁLOVÁ, J. VLAS, T. MAŠATA, D. Effect of the Ionizing Radiation Dose on Histone H2AX. In Proceedings of the International Conference Nuclear Energy for New Europe (NENE 2021). Ljubljana: Nuclear Society of Slovenia, 2021. s. 1015.1-1015.7. ISBN: 978-961-6207-51-5
Datum vydání:	2021
Nakladatel:	Nuclear Society of Slovenia
Typ dokumentu:	konferenční příspěvek ConferenceObject
URI:	http://hdl.handle.net/11025/46763
ISBN:	978-961-6207-51-5
Klíčová slova v dalším jazyce:	histone H2AX;ionizing radiation;gamma radiation;biodosimetry;flow cytometry
Abstrakt v dalším jazyce:	In case the radiation dosimeters are not available, biological dosimetry represents an important method to estimate the absorbed dose of the exposed individuals during nuclear events. Nevertheless, the eukaryotic DNA is constantly exposed to exogenous and endogenous factors. Apart from the ionizing radiation, widescale DNA lesions are also induced by other harmful effects. DNA double-strand breaks (DSBs) are the gravest lesions. DSBs provoke an extensive reaction characterized by the expression of the H2AX molecule. The scope of this work is an assessment of a gamma radiation dose-effect on a human body in terms of expression of the H2AX in DNA. This contribution is focused on the expression scale of the phosphorylated H2AX molecule (g-H2AX), which highlights a DNA damage induced by the exposure to gamma radiation. The dependency between the share of g-H2AX molecule in an irradiated sample and the radiation dose was examined. The investigated subjects consist of fourteen samples of uncoagulable blood from healthy donors. The sample of each donor was divided into four test tubes – a negative control + three levels of gamma radiation (0.5 Gray, 1 Gray, 2 Gray). The irradiation was performed on a medical caesium source “Gammacell® 1000 Elite.” The evaluation was based on the method for determining g-H2AX after chemical stimulation DNA – extracorporeal photopheresis. The outcome of this work is the confirmation that the production of this molecule is dependent on the dose of gamma radiation. Owing to the g-H2AX characteristic, the finding of the relation between the share of g-H2AX molecule in a sample and a dose of radiation was statistically confirmed. Accordingly, the H2AX molecule can be considered a reliable specific marker for DNA damage. In the future, this method could find a purpose in practical events, for example, re-determination dose of radiation after nuclear events.
Práva:	Plný text je přístupný v rámci univerzity přihlášeným uživatelům. © Nuclear Society of Slovenia
Vyskytuje se v kolekcích:	Konferenční příspěvky / Conference papers (KAZ) Konferenční příspěvky / Conference Papers (KEE) OBD

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