Title: Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry
Authors: Luptáková, Dominika
Pluháček, Tomáš
Petřík, Miloš
Novák, Jiří
Palyzová, Andrea
Sokolová, Lucie
Škríba, Anton
Šedivá, Blanka
Lemr, Karel
Havlíček, Vladimír
Citation: LUPTÁKOVÁ, D., PLUHÁČEK, T., PETŘÍK, M., NOVÁK, J., PALYZOVÁ, A., SOKOLOVÁ, L., ŠKRÍBA, A., ŠEDIVÁ, B., LEMR, K., HAVLÍČEK, V. Non-invasive and invasive diagnoses of aspergillosis in a rat model by mass spectrometry. Scientific Reports, 2017, roč. 7, č. NOV, s. 1-10. ISSN: 2045-2322
Issue Date: 2017
Publisher: Nature Publishing Group
Document type: článek
URI: 2-s2.0-85036560721
ISSN: 2045-2322
Keywords in different language: FUMIGATUS,SIDEROPHORES,GA-68-SIDEROPHORES
Abstract in different language: Invasive pulmonary aspergillosis results in 450,000 deaths per year and complicates cancer chemotherapy, transplantations and the treatment of other immunosuppressed patients. Using a rat model of experimental aspergillosis, the fungal siderophores ferricrocin and triacetylfusarinine C were identified as markers of aspergillosis and quantified in urine, serum and lung tissues. Biomarkers were analyzed by matrix-assisted laser desorption ionization (MALDI) and electrospray ionization mass spectrometry using a 12T SolariX Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. The limits of detection of the ferri-forms of triacetylfusarinine C and ferricrocin in the rat serum were 0.28 and 0.36 ng/mL, respectively. In the rat urine the respective limits of detection achieved 0.02 and 0.03 ng/mL. In the sera of infected animals, triacetylfusarinine C was not detected but ferricrocin concentration fluctuated in the 3-32 ng/mL range. Notably, the mean concentrations of triacetylfusarinine C and ferricrocin in the rat urine were 0.37 and 0.63 μg/mL, respectively. The MALDI FTICR mass spectrometry imaging illustrated the actual microbial ferricrocin distribution in the lung tissues and resolved the false-positive results obtained by the light microscopy and histological staining. Ferricrocin and triacetylfusarinine C detection in urine represents an innovative non-invasive indication of Aspergillus infection in a host.
Rights: © Nature Publishing Group
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