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dc.contributor.authorOstašov, Pavel
dc.contributor.authornull, null
dc.contributor.authorRobertson, Henry
dc.contributor.authorPiazza, Paolo
dc.contributor.authorDatta, Avik
dc.contributor.authorApperley, Jane
dc.contributor.authorHoudová, Lucie
dc.contributor.authorLysák, Daniel
dc.contributor.authorHolubová, Monika
dc.contributor.authorTesařová, Kateřina
dc.contributor.authorCaputo, Valentina S.
dc.contributor.authorBarozzi, Iros
dc.date.accessioned2021-03-15T11:00:27Z-
dc.date.available2021-03-15T11:00:27Z-
dc.date.issued2020
dc.identifier.citationOSTAŠOV, P. ROBERTSON, H. PIAZZA, P. DATTA, A. APPERLEY, J. HOUDOVÁ, L. LYSÁK, D. HOLUBOVÁ, M. TESAŘOVÁ, K. CAPUTO, VS. BAROZZI, I.Evolution of Advanced Chronic Lymphoid Leukemia Unveiled by Single-Cell Transcriptomics: A Case Report. Frontiers in Oncology, 2020, roč. 10, č. OCT 2020, s. 1-9 (article 584607). ISSN 2234-943X.cs
dc.identifier.issn2234-943X
dc.identifier.uri2-s2.0-85096052038
dc.identifier.urihttp://hdl.handle.net/11025/42923
dc.format9 s.cs
dc.format.mimetypeapplication/pdf
dc.language.isoenen
dc.publisherFrontiers Mediaen
dc.relation.ispartofseriesFrontiers in Oncologyen
dc.rights© Frontiers Mediaen
dc.titleEvolution of Advanced Chronic Lymphoid Leukemia Unveiled by Single-Cell Transcriptomics: A Case Reporten
dc.typečlánekcs
dc.typearticleen
dc.rights.accessopenAccessen
dc.type.versionpublishedVersionen
dc.description.abstract-translatedGenetic and transcriptional heterogeneity of Chronic lymphocytic leukaemia (CLL) limits prevention of disease progression. Longitudinal single-cell transcriptomics represents the state-of-the-art method to profile the disease heterogeneity at diagnosis and to inform about disease evolution. Here, we apply single-cell RNA-seq to a CLL case, sampled at diagnosis and relapse, that was treated with FCR (Fludarabine, Cyclophosphamide, Rituximab) and underwent a dramatic decrease in CD19 expression during disease progression. Computational analyses revealed a major switch in clones’ dominance during treatment. The clone that expanded at relapse showed 17p and 3p chromosomal deletions, and up-regulation of pathways related to motility, cytokine signaling and antigen presentation. Single-cell RNA-seq uniquely revealed that this clone was already present at low frequency at diagnosis, and it displays feature of plasma cell differentiation, consistent with a more aggressive phenotype. This study shows the benefit of single-cell profiling of CLL heterogeneity at diagnosis, to identify clones that might otherwise not be recognized and to determine the best treatment options.en
dc.subject.translatedchronic lymphoid leukemia (CLL)en
dc.subject.translatedsingle-cell RNA-seq (scRNA-seq)en
dc.subject.translatedtherapy resistanceen
dc.subject.translateddisease progressionen
dc.subject.translatedadvanced diseaseen
dc.subject.translatedcase reporten
dc.identifier.doi10.3389/fonc.2020.584607
dc.type.statusPeer-revieweden
dc.identifier.document-number589084000001
dc.identifier.obd43930592
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