Title: Diet Rich in Animal Protein Promotes Pro-inflammatory Macrophage Response and Exacerbates Colitis in Mice
Authors: Kostovcikova, Klara
Coufal, Stepan
Galanova, Natalie
Fajstova, Alena
Hudcovic, Tomas
Kostovcik, Martin
Prochazkova, Petra
Jiraskova Zakostelska, Zuzana
Cermakova, Martina
Šedivá, Blanka
Kuzma, Martin
Tlaskalova-Hogenova, Helena
Kverka, Miloslav
Citation: KOSTOVCIKOVA, K., COUFAL, S., GALANOVA, N., FAJSTOVA, A., HUDCOVIC, T., KOSTOVCIK, M., PROCHAZKOVA, P., JIRASKOVA ZAKOSTELSKA, Z., CERMAKOVA, M., ŠEDIVÁ, B., KUZMA, M., TLASKALOVA-HOGENOVA, H., KVERKA, M. Diet Rich in Animal Protein Promotes Pro-inflammatory Macrophage Response and Exacerbates Colitis in Mice. Frontiers in Immunology, 2019, roč. 10, č. 26 April 2019, s. 1-14. ISSN 1664-3224.
Issue Date: 2019
Publisher: Frontiers Media
Document type: článek
URI: 2-s2.0-85066948088
ISSN: 1664-3224
Keywords in different language: dietary protein;microbiota, colitis;germ-free;macrophage
Abstract in different language: Diet is a major factor determining gut microbiota composition and perturbances in this complex ecosystem are associated with the inflammatory bowel disease (IBD). Here, we used gnotobiotic approach to analyze, how interaction between diet rich in proteins and gut microbiota influences the sensitivity to intestinal inflammation in murine model of ulcerative colitis. We found that diet rich in animal protein (aHPD) exacerbates acute dextran sulfate sodium (DSS)-induced colitis while diet rich in plant protein (pHPD) does not. The deleterious effect of aHPD was also apparent in chronic DSS colitis and was associated with distinct changes in gut bacteria and fungi. Therefore, we induced acute DSS-colitis in germ-free mice and transferred gut microbiota from aCD or aHPD fed mice to find that this effect requires presence of microbes and aHPD at the same time. The aHPD did not change the number of regulatory T cells or Th17 cells and still worsened the colitis in immuno-deficient RAG2 knock-out mice suggesting that this effect was not dependent on adaptive immunity. The pro-inflammatory effect of aHPD was, however, abrogated when splenic macrophages were depleted with clodronate liposomes. This treatment prevented aHPD induced increase in colonic Ly-6Chigh pro-inflammatory monocytes, but the ratio of resident Ly-6C−/low macrophages was not changed. These data show that the interactions between dietary protein of animal origin and gut microbiota increase sensitivity to intestinal inflammation by promoting pro-inflammatory response of monocytes.
Rights: © Frontiers Media
Appears in Collections:Články / Articles (KMA)

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Please use this identifier to cite or link to this item: http://hdl.handle.net/11025/34865

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