Title: CDTE quantum dots – preparation, physico-chemical properties and application as fluorescent markers
Authors: Hezinová, V.
Svobodová, I.
Lišková, M.
Přikryl, J.
Klepárník, K.
Foret, F.
Citation: Electroscope. 2009, Konference EDS 2009.
Issue Date: 21-Nov-2009
Publisher: Západočeská univerzita v Plzni, Fakulta elektrotechnická
Document type: článek
konferenční příspěvek
article
conferenceObject
URI: http://147.228.94.30/images/PDF/Rocnik2009/EDS_2009/svobodova.pdf
http://hdl.handle.net/11025/533
ISSN: 1802-4564
Keywords: CdTe kvantové body;fluorescenční značky;fyzikálně-chemické vlastnosti
Keywords in different language: CdTe quantum dots;fluorescent markers;physicochemical properties
Abstract in different language: A set of water-soluble CdTe quantum dots (QDs) was prepared by the chemical reaction between cadmium chloride and sodium hydrogen telluride in the presence of 3-mercaptopropionic acid (MPA). QDs were characterized using fluorescence spectroscopy, lifetime measurements, slab-gel and capillary electrophoresis. The particle sizes, determining the wavelength of the fluorescence emission, were evaluated to be from 2.5 nm (500 nm) to 5.2 nm (750 nm). The fluorescence lifetimes determined by the time-resolved fluorescence spectrometry, increases from 3.05 to 20.5 ns with the increasing size of particles. The uptake of non-conjugated QDs into the living cells, which has a potential for single cell analyses, was apparent for human lymphocytes and yeast cells (Saccharomyces cerevisiae) after 30 minutes and 3 hours, respectively. The cell manipulation, lysis and monitoring was performed in microfluidic devices with laser-induced fluorescence detection. The conjugation of QDs with functional biomolecules allows the selective labeling and localization of specific molecules. Several coupling agents such as N-hydroxysulfosuccinimide (NHS) and 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride (EDC) or carbonyldiimidazole (CDI) were used. The concentrations of reaction products were checked by capillary electrophoresis with laser-induced fluorescence detection.
Rights: Copyright © 2007-2010 Electroscope. All Rights Reserved.
Appears in Collections:Konference EDS 2009
EDS 2009 (2009)

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